Fluorescence and Raman Spectroscopy for
Scaled-Down in Situ Product Monitoring 

Member: North Carolina State University  

This project will evaluate scale-down approaches in microbioreactors and their applicability in predicting deployment of process analytical technology and fermentation performance throughout scale-up. Uni- and multivariate data analysis will be used to compare process data and key fermentation metrics, as well as generate batch-level models for inline monitoring of recombinant protein product and cell density monitoring via Raman spectroscopy.  

Developing successful scale-down approaches will allow for reduced material use, including medium reagents, process gases, high-purity water and energy used to create clean steam, as well as cleaning reagents. The ability to predict commercial production challenges using microbioreactors will also reduce cost and development time associated with typical scale-up efforts. 

Year launched: 2023 

Funding source: BioMADE Project Call 3.0