• Betamethylvalerolactone Downstream Processing and Application Development
  Check out the journey of a team of BioMADE members led by Valerian Materials who worked together to develop fermentation, downstream processing, and catalytic conversion methods that transformed a shake‑flask production of mevalonate in E. coli into a 270‑L, optimized, high‑tier production and purification process for a fully recyclable, highly versatile platform polymer Betamethylvalerolactone (BMVL).

• Optimizing Feedstock Utilization; Sensing and Automation; and Separation
  Learn about the eVOLVER platform, a continuous culture platform enabling individual control of 16 different experiments simultaneously, find information on wireless sensor integration with bioreactors, and a report on protocols and membranes for separation.

• Rational Fermentation Scaleup: Response of Metabolic Kinetics to Hydrodynamic Variability
  In this report, learn how Computational Fluid Dynamics (CFD) can support risk‑reduction in scale‑up and find information on the capabilities and data infrastructure needed for broader industrial adoption.

• Simulating Manufacturing-Scale Aerobic Fermentation
  Find data and findings from a project that evaluated the suitability of the H.E.L. BioXplorer 400P bioreactor system for simulating scaled-up fermentation bioprocess conditions.   

• Report: Alkaline Isolation of Insoluble Proteins from Biomass
  An overview of improving and scaling downstream processing for the isolation of insoluble, material-forming proteins from bacterial biomass. Includes an overview of the process steps and the major process parameters that should be evaluated when implementing this method.   

• Report: Cost Effective Production of Healthy and Sustainable Oils and Fats
  Find protocols on how to perform different measurements for oil concentration in your samples, including total Triacylglycerols (TAG) in cell suspension, free TAGs and the concentration of oil phase in the top layer of your purified samples. 

• Report: Efficiencies Gained by Transitioning from a Batch to a Continuous Downstream Process of Biobased Dimethylmalonate
  Find a case study, technical data sheets, a report on batch vs. continuous systems, and a report on the impact of recycling process streams.

• Report: Upscaling and Downscaling Purification for a Novel Bioplastic Process
  Access data and results on DSP performance at bench and pilot scale, beneficial strain modifications, and a TEA and fermentation process summary for the bioplastic polymer P3HP (poly-3-hydroxypropionate).

• Report: Plasmid Construction in E. coli
  Plasmid instability is a critical concern for high-intensity plasmid-based biomanufacturing processes using E. coli . In this brief report, BioMADE member Tandem Repeat describes how they successfully reduced plasmid concatemerization in E. coli, one important mechanism causing plasmid instability, via genetic engineering.

• Gas Fermentation to Enable Decentralized Local Biomaterials Manufacturing
  Find resources from this project including a TEA model on optimal plant sizing, a life cycle model comparing uses of biogas, a poster on methane to PHA production, and The Roadmap to Biomanufacturing presentation for building your facility.

• Industry report and open-source software instructions
  Find instructions for running BlueSTEAM, an open-source code base that simplifies the techno-economic analysis for anaerobic biomanufacturing processes.

• Joint Integrated Modeling for Systematic Optimization of eNvironmental Impacts and Costs (JIMSONIC)
  Access resources that enable BioMADE members to conduct rapid, affordable techno‑economic (TEA) and life cycle analyses (LCA) from early bench work onward, including analysis tools, Excel models, a user guide, and instructional videos.

• Best Practices for Benchmarking Product Performance
  Review these best practices to set and track achievable metrics to accelerate product development, shorten time to manufacturing readiness, and reduce costs.

• Fluorescence and Raman Spectroscopy for Scaled-Down in Situ Product Monitoring
  Access a microbioreactor data set, a report on a validated scale-down process, Raman spectra datasets with accompanying process data, batch-level multivariate models for each of the datasets, and technical bulletins on Raman and scale-down.

• Report on HAZOPS Approach, Safety Considerations, and Best Practices for Piloting 
  Learn about the safety practices put in place while pilot continuous production of biobased DMM included hazards and operability, control strategy and interlock matrix, SOPs, and equipment commissioning.

BioMADE’s Digital Backbone is available to members as a beta launch. The BioMADE Digital Backbone is a bioprocess data management platform that enables organizations to build a standardized, centralized source of truth. By making your data AI-ready, the Backbone empowers faster, more confident decision-making at every level of your organization. Learn more here.